FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47631877
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1557760.32704148946303335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT283300.026.79482745
AGACCGC303650.025.5400284
CAGACCG311300.024.99193
ACCGCGT326350.023.7359096
GACCGCG327250.023.6775555
CCGCGTT327600.023.6453917
CGCGTTC331100.023.4158258
AGAACGA331200.023.2388844
TCAGACC358050.022.11842
GCGTTCT362300.021.4614359
TACGGAG355300.021.3975334
ATACGGA358250.021.33433533
AATACGG359600.021.28530532
CTCAGAC398300.021.1784571
AAGAACG370100.020.81455243
CAATACG377900.020.34385731
ACGGAGA379850.020.11528635
CCCAATA396350.019.46548529
CGGAGAG396900.019.137836
CGTTCTC424150.018.61834110
Produced by FastQC (version 0.11.5)