FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47631877
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG22850.014.46783426
AGCTTAG132450.014.10684145
GGCATAG139700.013.27614635
ATAGGGG143900.013.13578638
ACCCGGC80750.012.3748145
GAGCTTA156100.011.91258844
CATAGGG160500.011.78009137
ATAGCTC81550.011.67201339
TGGCATA162950.011.64452434
GGGAGGT243100.011.5755743
ACTCTCC238550.010.96281911
GCATAGG173150.010.88047536
GGTCACT239000.010.8378767
GGAGGTC258950.010.49284654
GTCACTC253750.010.2788958
TCAGACG84800.010.1691862
TGTCGTT32650.010.05904228
CCGGACA109200.010.05755112
CACCCGG104350.010.0529944
TCACCCG104400.010.0485383
Produced by FastQC (version 0.11.5)