FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46361502
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1055090.22757890803451536No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG832000.17945924185113762No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT791830.17079472533051238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC199300.020.0126294
CAGACCG210000.019.2178973
GACCGCG209850.019.1885875
AACGCTA325600.019.04025523
ACGCTAC327250.018.95115324
ACCGCGT217650.018.521116
CGCTACC336400.018.40891825
AAAACGC352100.017.68032621
TTCACGC361400.017.6152327
TCACGCT359450.017.5856278
AAACGCT356350.017.49199722
CCGGCTT357900.017.4234522
GAAAACG360350.017.28576320
GCCGGCT308850.017.0131191
ACGCTCA380600.016.82116910
TTCGACG19000.016.6937246
TCAGACC250000.016.5569572
CCGCGTT241350.016.5440247
CGCGTTC246250.016.2513628
CGGCTTC408000.015.9676533
Produced by FastQC (version 0.11.5)