FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46361502
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG551550.11896724139783046No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT500460.10794732232790903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG29500.014.11845926
GTTCTAT186750.013.2457691
ATAGGGG163850.011.54834338
AGGGATC165350.011.5190572
GGAGGTC226400.011.502074
GGCATAG162350.011.46103535
AGCTTAG172200.011.06245345
GGGAGGT244100.011.0096493
GGTCACT229950.010.8289927
GTCACTC233700.010.6933488
TGGCATA177500.010.645246534
TGTCGTT39150.010.6388928
GAGCTTA179950.010.50992944
ATATAGC49850.010.4686466
TCAGACG84050.010.2323742
TTCTATA505600.010.0991952
TCTATAG498900.010.0110763
ACTCTCC250900.09.93031611
CAGGGAT218400.09.6949181
GACGCAA47200.09.58796544
Produced by FastQC (version 0.11.5)