FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50791412
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT935730.18422996391594704No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG740360.14576479976575568No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA577990.11379679698607316No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC518270.10203890374223107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG28850.024.7998896
GACCGCG198350.022.8342325
ACCGCGT199000.022.7819236
AGACCGC205600.022.1167564
GAACGAT206500.021.87822345
CAGACCG212900.021.4966853
CGCGTTC213350.021.2706958
CCGCGTT214500.021.1251897
CCGGCTT248600.018.4096722
TCACGCT224050.018.387048
TTCACGC225700.018.3523067
TCAGACC258950.017.9866662
TCGACGA40800.017.9773587
AGAACGA253750.017.94621544
GCCGGCT221650.017.573061
GCGTTCT260500.017.5589319
AATACGG263850.017.22513632
AACGCTA229200.017.21005823
GTTCGAC40700.017.1925725
CGGTATA51650.017.1210520
Produced by FastQC (version 0.11.5)