FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50936110
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1084490.21291182228089267No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG815160.16003577815424067No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG749570.14715886234736025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG45050.016.23082426
ATATAGC62300.014.1895496
GGGAGGT370250.013.7837873
GGAGGTC377150.013.3530274
GCTAAGT58750.013.09589614
GTCGTTC59300.012.78664629
TGTCGTT60550.012.59705828
GAGGTCA414300.012.3896555
AGCTTAG329350.012.11757445
AACTCTA119550.012.01256445
GGTCACT417350.011.7447077
GTCACTC422300.011.5699938
CACCGAA497350.011.43235945
TATAGCT75800.011.3661927
AGGTCAC451250.011.2755956
GTCGCCG29750.011.19797144
TCGTTCC66550.011.12330830
ACTCTCC456300.011.00407311
ATAGGGG369800.010.93475638
GGCATAG360500.010.92359235
Produced by FastQC (version 0.11.5)