FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45592050
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG137300.012.49212745
ATAGGGG148700.011.77510738
GGGAGGT256050.011.4048843
GGCATAG148900.011.37979835
GAGCTTA163900.010.46471944
GGAGGTC266300.010.3501094
GTCACTC262500.010.2778178
TGGCATA170350.010.09162734
GGTCACT275300.09.9307277
TAGGGGA277650.09.5324939
CATAGGG182100.09.52835237
ACTCTCC287300.09.42977511
TCACTCT290100.09.3930659
ACCCGGC69650.09.1102225
CCGCGTT28450.09.09455113
CGCGTTC29150.09.03006914
GCGTTCG28800.08.90472315
GCATAGG194500.08.79320736
AGCATAG106750.08.75048645
AGGTCAC336750.08.6798556
Produced by FastQC (version 0.11.5)