FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46659726
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC574010.12302043951136789No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG507140.10868902230587467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA135400.020.50425123
ACGCTAC136550.020.2988124
CGCTACC143550.019.3096125
AAAACGC152000.018.3382721
TCACGCT164900.017.5717548
GCCGGCT167900.017.4224451
GAAAACG160550.017.41746720
TTCACGC170750.017.2200667
CCGGCTT183800.016.9918692
AAACGCT168550.016.7378422
ACGCTCA177250.016.57590910
GTTCCTA86100.015.93848715
GTCCGTT31600.015.8203661
AGACCGC120150.015.61850554
ACCGCGT121300.015.486266
CGGCTTC211850.015.2731143
CACGCTC191350.015.2251399
GACCGCG125450.014.9407215
CGTCGCA99450.014.92997557
CCGCGTT127450.014.7212997
Produced by FastQC (version 0.11.5)