FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46659726
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC56700.011.2286175
AGCTTAG123450.011.02871545
GGCATAG130250.010.26233435
ATAGGGG138550.09.95608238
CGGGACG55700.09.57337117
GGGAGGT224500.09.5099423
ATAGCTC60250.09.33704939
TGGCATA147950.09.30836434
CATAGGG145550.09.29181537
TCGCGCT25200.09.28738545
GGAGGTC214900.09.2437684
ATAACGC71050.09.21627839
GTTGTCG17000.09.131989526
CGTCAGT91600.09.0139251
ACGGACA67400.09.01312918
GAGCTTA150650.08.9925444
TAACGCC74900.08.89331240
TCACCCG78900.08.8677073
GCATAGG157650.08.66427236
CCGGACA75900.08.4775712
Produced by FastQC (version 0.11.5)