FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48694127
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1280940.2630584177019952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT279150.022.14278845
GACCGCG324900.018.9479875
AGACCGC327300.018.905264
ACCGCGT326250.018.8764786
CAGACCG332050.018.655143
CCGCGTT340950.018.0494277
CGCGTTC343050.017.9651628
AGAACGA347800.017.79785244
TCAGACC379800.016.7065262
CGTTACG18150.016.6333221
GCGTTCT388300.015.9990549
CTCAGAC438300.015.5901791
AATACGG402850.015.32220132
TTCGACG30850.015.2380426
ATACGGA408100.015.14730733
TACGGAG407850.015.14007834
AAGAACG411550.015.00810143
CAATACG419800.014.74105531
ACGGAGA433100.014.2413835
CCGTACG55900.014.0829714
Produced by FastQC (version 0.11.5)