FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48694127
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG31800.012.80377626
AGCTTAG149950.011.54412345
ATAGGGG159500.011.09026838
GGCATAG159300.010.91907835
GGGAGGT241100.010.7769513
GGAGGTC233400.010.487444
GAGCTTA173400.010.04784444
CATAGGG177900.09.80405937
TGGCATA178500.09.7065934
GTCACTC248350.09.63168
GGTCACT247350.09.6250237
TGTCGTT44800.09.49098828
ATATAGC52900.09.4858226
ACTCTCC258400.09.41374711
GCATAGG187600.09.26098336
TATAGCT55550.08.9928417
GAGGTCA291400.08.8185195
AGGGATC142700.08.6702932
ACCCGGC71950.08.5066235
TTGTCGT58500.08.30596827
Produced by FastQC (version 0.11.5)