FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47065970
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC68250.013.0266795
GGCATAG123150.012.60241835
GTTGTCG18250.012.09670426
GGGAGGT194100.012.0491293
AGCTTAG128550.012.02797445
ATAGGGG130100.011.91153538
TCACCCG75850.011.7518233
GGAGGTC188700.011.4989034
GAGCTTA138850.011.18253544
TGGCATA145550.010.86358334
CACCCGG87200.010.4283644
CCGGACA85550.010.19891212
CATAGGG151900.010.05409337
GCATAGG158350.09.815236
ACGGACA94000.09.61687218
ATAGCTC84000.09.58691839
GGTCACT219100.09.4962867
TAGCGTA6950.09.38521237
CGGCCCG94700.09.3345488
CGGACAG90150.09.17873219
Produced by FastQC (version 0.11.5)