FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48447478
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1108520.22880860795271943No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG719010.148410202074915No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG493110.10178238792946043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC248800.023.2424034
CAGACCG258250.022.5298793
GACCGCG269100.021.4038265
ACCGCGT278900.020.7645686
CCGCGTT295850.019.5669847
CGCGTTC298600.019.401498
TTCGACG23550.019.298496
TCAGACC309950.019.0626012
GCGTTCT308000.018.7796429
CTCAGAC343850.018.6444851
GAACGAT327050.017.0583845
CCGTACG58600.016.5108384
CTACACG160400.016.00872245
CGTTCTC372250.015.8767410
GCCGGCT254150.015.8541281
CGAACGA91000.015.55316432
CGTACGC61850.015.5329275
CGGTATA43250.015.50031720
CCCGTAT33500.015.4547671
TCGACGA29800.015.1752387
Produced by FastQC (version 0.11.5)