FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48447478
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA674080.1391362415191148No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG577970.1192982635752474No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG542210.11191707440374914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG198200.014.90007545
GGCATAG201450.014.60755735
ATAGGGG208100.014.38945438
GGGAGGT312250.014.2050913
GGAGGTC306200.013.70342354
GAGCTTA221600.013.35670944
TGGCATA229350.012.903334
GGTCACT319250.012.8281387
GTCACTC326650.012.6688258
ACCCGGC75600.012.6120985
ACTCTCC328450.012.49050711
GAGGTCA344100.012.3449845
GTTGTCG19500.012.12397626
AGGTCAC357450.011.8013966
CATAGGG254450.011.60932437
TCACTCT365400.011.5901079
CACTCTC357300.011.48207610
TCACCCG88500.011.4565193
TGGGAGG276100.011.406282
GCATAGG269800.010.97391636
Produced by FastQC (version 0.11.5)