FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50948032
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT988340.19398982869446263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC212250.021.7847734
GACCGCG217050.021.3237425
CAGACCG223000.021.0272143
ACCGCGT224000.020.6822226
GAACGAT225550.020.51965945
TTCGACG28500.020.447676
CGCGTTC230950.020.0576088
CCGCGTT233900.019.8142537
TCAGACC262000.018.103322
AGAACGA277150.016.7315244
AATACGG281450.016.45086332
ATACGGA284200.016.38667533
TACGGAG284200.016.30751234
CTCAGAC327150.015.9376131
CGTTACG18400.015.65066721
CAATACG298100.015.59994631
AAGAACG299600.015.55926743
TCGACGA39100.015.478057
CCGTACG70250.015.1817184
CGTACGC71900.014.9272015
Produced by FastQC (version 0.11.5)