FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51453186
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1296070.25189305089873343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT250500.024.77166445
AGACCGC275250.022.6104894
ACCGCGT282900.021.9990736
GACCGCG283700.021.889455
CAGACCG286950.021.8287853
CCGCGTT291800.021.3126727
CGCGTTC294300.021.1545628
AGAACGA304900.020.36783244
TCAGACC333200.019.0756242
GCGTTCT335150.018.6365099
AATACGG337000.018.34075532
TACGGAG340850.018.08078434
ATACGGA343350.017.96879233
CTCAGAC390000.017.878971
AAGAACG357900.017.36421443
CAATACG359300.017.30234531
ACGGAGA362200.017.01500535
CGGAGAG368900.016.62058636
CGTTCTC395650.016.06535510
GTTCCTA95900.015.97691515
Produced by FastQC (version 0.11.5)