FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46619105
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT574980.12333570110365696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC124800.023.2568974
CAGACCG130950.022.2505593
GACCGCG138850.021.0656155
ACCGCGT144650.020.2209516
CCGCGTT149350.019.5996677
GAACGAT153650.019.00722945
CGCGTTC157200.018.6066178
AATACGG173300.016.72222132
ATACGGA174350.016.69894433
TACGGAG173600.016.69332334
TCAGACC178900.016.6515372
AGAACGA176800.016.50571644
GCGTTCT180400.016.3634159
CGTTACG22650.015.7944921
CCGTACG67350.015.5009054
CGTACGC71900.014.8954875
GTCCGTT35800.014.8411951
CTCAGAC235200.014.8270241
CAATACG199850.014.74836331
AAGAACG202850.014.48587743
Produced by FastQC (version 0.11.5)