FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47298481
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT7505601.5868585716315073No Hit
GCCGGCTTCACGCTCAGGAGAAAAACGCTACCTCTCTTCCTCGTGGTTTTC1835680.388105486939422No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1739060.3676777696095568No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1334820.28221202283430624No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC781940.16532031969483332No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG698550.14768973236159527No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGT580490.12272909990492084No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG563470.11913067567645566No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC538590.11387046446586732No Hit
CTCAGGAGAAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC534160.11293385933472154No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC533350.1127626064777852No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTA522470.11046232118955364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT1198850.030.40347745
CAGACCG1389950.026.4940513
AGACCGC1394050.026.4048884
TCAGACC1431950.026.0543542
AGAACGA1407200.025.9354444
GACCGCG1415700.025.8358065
ACCGCGT1430500.025.5779486
CTCAGAC1487150.025.5655021
CCGCGTT1449950.025.2332847
CGCGTTC1455650.025.1313868
GCGTTCT1469350.024.9353459
CGTTCTC1566750.023.68924510
AAGAACG1562100.023.36220443
AATACGG1561550.023.32594332
CCTCTCA1588600.023.31609319
ACTCCCC1586750.023.27048525
ATACGGA1564250.023.26410933
TACGGAG1560700.023.22476634
TCACTCC1605350.023.11327423
CCCCAAT1597250.023.0539628
Produced by FastQC (version 0.11.5)