FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52394681
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1946880.371579702909156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT350600.026.43879945
AGACCGC397200.023.5298334
CAGACCG406150.023.1054953
GACCGCG425800.021.9757655
AGAACGA421500.021.96486944
ACCGCGT428900.021.9165956
CCGCGTT438700.021.4421487
CGCGTTC441500.021.2857788
TCAGACC456750.020.7933122
GCGTTCT455200.020.595729
AAGAACG461400.020.04590643
AATACGG474700.019.56101232
ATACGGA475850.019.51373933
TACGGAG475900.019.4777234
CTCAGAC515900.019.3760191
CAATACG490950.018.98688731
ACGGAGA502900.018.4812735
TTCGGCG78150.018.25669344
CGGAGAG513200.018.07123636
CCCAATA520650.017.92108229
Produced by FastQC (version 0.11.5)