FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43084758
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG666230.15463241084004695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG197150.017.05059845
GGCATAG229250.014.7415435
GAGCTTA233250.014.5177644
ATAGGGG236800.014.366528538
GGGAGGT348200.013.9963243
TGGCATA245300.013.82286234
GTTGTCG27950.013.76562926
GGAGGTC348250.013.535594
CATAGGG265300.012.848639537
ACTCTCC363550.012.74927111
GCATAGG277350.012.27419536
GGTCACT396750.011.5860057
GTCACTC402300.011.5827548
CACTCTC405750.011.42882910
TGGGAGG322100.011.0998592
TCTCCCC423050.011.06251613
TAGGGGA420500.011.01722239
GCTCTGT429100.010.95372122
AGCATAG115350.010.86484245
AGGGGAG436850.010.77999740
Produced by FastQC (version 0.11.5)