FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50466922
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTC508930.10084427181828129No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAA508220.10070358560801469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC61700.017.4307144
GTCCGTT30700.017.2300511
CAGACCG67600.016.1423763
AGACGCT198250.016.00451341
CGGATAC77200.016.0002773
ATGTCGC201300.015.80762835
TTCGGCG72800.015.79354544
CCGTACG75050.015.40936854
GAACGAT70450.015.29774345
CCCGGAT131550.015.2969111
AACGCTA63700.015.25833923
CCGCAGA216350.014.94756437
CCCGTAT25850.014.889931
CCCCGCA208300.014.85506435
CGTACGC79400.014.8201555
ACGCTAC66500.014.75156624
TGTCGCT218650.014.67711236
CTCGGTT74050.014.6513971
CGTTACG11250.014.59880621
AAGCGGC207300.014.40343228
Produced by FastQC (version 0.11.5)