FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56776582
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT890050.15676357551780767No Hit
CTTTATTTTGATGAGAATTTTTAGTCTCACGTTGTTGTTTGTTAGCTACTA592480.10435288267264839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT179750.023.85772345
TTCGACG40200.023.5067256
AGACCGC220550.021.1478614
CAGACCG225400.020.812623
CCGCGTT227650.020.3694447
CGCGTTC227950.020.3525098
GACCGCG227750.020.3210755
ACCGCGT227950.020.3031586
CGGTATA62650.019.50324620
GTTCGAC50600.018.6753835
CGACGAG49850.018.2792648
AATACGG253200.018.21625132
AGAACGA237950.018.19234344
ATACGGA254750.018.0700933
TCGACGA53200.018.0163527
TACGGAG258200.017.74150334
TCAGACC273500.017.5060792
CGTTACG14800.017.48508521
TTAGTCT235550.017.19574521
TTTAGTC236500.016.9362420
Produced by FastQC (version 0.11.5)