FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56776582
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG164300.014.01159145
ATAGGGG174700.013.26592438
GGCATAG174600.013.12962635
GTTGTCG27850.013.00425826
GGGAGGT277000.012.2218923
TGGCATA196400.011.76376534
GAGCTTA198800.011.693244
GGTCACT274200.011.6530227
GTCACTC276800.011.5192588
ACTCTCC284250.011.32798511
CATAGGG204500.011.31009337
GGAGGTC302100.011.0423764
GCATAGG217150.010.702270536
GAGGTCA322150.010.5087365
TCAGACG90300.010.020982
ATATAGC55300.09.8070656
GGAACAT341350.09.6547411
CATTCAA312900.09.5892665
CAAGTAA285900.09.5548979
TGCGCAC163050.09.49366837
Produced by FastQC (version 0.11.5)