FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50252603
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT730090.14528401643194483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG18350.021.7010156
GAACGAT170250.021.08248945
AGACCGC180800.020.5692144
CAGACCG190300.019.767023
AGAACGA206850.017.37391544
ATACGGA218650.016.24894533
TACGGAG218300.016.15020434
CCGTACG63900.016.1253034
GCGTTCT233250.016.0403029
AATACGG223150.015.9414432
GACCGCG236200.015.8304785
CGTACGC66150.015.7808725
TTCGGCG64750.015.70894344
CTCAGAC281500.015.4431991
CGACGAG25200.015.0878788
CGGTATA31250.015.04700120
TCAGACC259750.014.8369812
CAATACG244000.014.80073831
ACGGAGA241750.014.71421835
TCGACGA27400.014.6154247
Produced by FastQC (version 0.11.5)