FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54248712
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1103030.20332832971223352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT214450.024.95978545
GACCGCG219200.024.2363225
ACCGCGT220700.024.1225786
AGACCGC227200.023.5314884
CCGCGTT229900.023.1945027
CGCGTTC230800.023.143058
CAGACCG239600.022.3700143
AGAACGA254050.021.12232244
TCAGACC284350.019.332392
AATACGG280650.019.08024632
GCGTTCT284250.018.9020779
TACGGAG289750.018.42686834
ATACGGA292250.018.35392833
CAATACG299700.017.86743531
CTCAGAC342900.017.7131731
AAGAACG304650.017.6361943
ACGGAGA306300.017.41653835
CGGAGAG318500.016.69995536
TTCGGCG67350.016.50295844
TTCGACG20050.016.3851266
Produced by FastQC (version 0.11.5)