FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48845895
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG599220.12267561071406309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA158800.020.03391523
GTTCCTA106950.019.64878315
ACGCTAC163450.019.50540424
CGCTACC167200.019.0006525
CCTACTC114100.018.69347618
CCGGCTT198100.018.5358452
TCACGCT181350.018.4360268
TTCACGC180800.018.3676667
GCCGGCT168150.018.3455181
AAAACGC176850.018.07819621
GTCCGTT39200.017.4619921
GAAAACG183600.017.43806320
AAACGCT188700.017.01449222
ACGCTCA198200.016.94814710
CCGTACG58400.015.9501294
AGTTACC139150.015.24808834
CGTACGC60500.015.2105415
CTACTCT138950.015.18838619
CGGCTTC258750.015.1476233
CCCGGAT122100.015.1402741
Produced by FastQC (version 0.11.5)