FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50043364
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT889830.17781178739302977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33750.027.3319876
AGACCGC180500.022.8627684
GAACGAT183550.022.54179445
CAGACCG188400.022.0474053
CGGTATA55250.022.03072220
TCGACGA45150.020.430897
CGACGAG45550.020.103298
GACCGCG217450.019.0087815
ACCGCGT230800.018.0049636
ATACGGA229950.017.9737133
AGAACGA231700.017.93502444
TACGGAG229600.017.9324834
AATACGG232350.017.81710432
CCGCGTT237600.017.470737
GTTCGAC53900.017.157645
CGCGTTC244900.016.986718
CTCAGAC280400.016.7686731
TCAGACC252750.016.7238942
CAATACG249000.016.67993431
AAGAACG251600.016.54330343
Produced by FastQC (version 0.11.5)