FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50043364
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG585090.11691660057065707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG198950.014.96234945
GGCATAG209800.014.0277635
ATAGGGG215400.013.861238
GGGAGGT305050.013.8500083
GTCACTC288250.013.6572148
GAGCTTA224350.013.298544
GGTCACT299100.013.1915587
AGGTCAC316350.012.87326056
ACTCTCC312150.012.73463811
GTTGTCG23950.012.49448326
TGGCATA239200.012.47294134
GAGGTCA330900.012.3938245
GGAGGTC334400.012.3382074
TCACTCT331500.012.1333899
CACTCTC330200.011.94286910
CATAGGG253950.011.739437
GCATAGG265200.011.20749136
TGGGAGG291000.011.10761452
AACTCTA99500.010.98998545
TAGCGTA8550.010.52621337
Produced by FastQC (version 0.11.5)