FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50828190
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG23850.019.0373746
ACCGCGT102350.018.952526
AGACCGC105650.018.444234
GACCGCG105450.018.3938565
CAGACCG115900.017.1626173
CCGCGTT116600.016.6362827
CGCGTTC117450.016.5345348
CGTTACG23650.016.25262521
TCGACGA30350.015.2564177
TCACGCT209250.015.1348038
ACGTTCG19850.014.9471186
CGAACGA45350.014.87683932
TCGGCGC86050.014.40094645
GCCGGCT226300.014.2768941
ACGGTAC118850.014.16121845
AACGCTA212100.014.13895523
ACGCTAC212500.014.11329824
CGCTACT75550.014.04556325
GTTCCTA76800.013.90156215
TACGGTA120500.013.87860244
Produced by FastQC (version 0.11.5)