FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50828190
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG569360.11201657977590782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC101450.014.502665
GGGAGGT368100.012.8649553
AGGTCAC332650.012.7886886
GGCATAG224200.012.72894435
GGTCACT326100.012.7282167
GGAGGTC336500.012.6958534
AGCTTAG226550.012.60678645
TCACCCG123350.012.5661453
GTCACTC333600.012.516248
GAGGTCA345850.012.3721365
ACTCTCC354050.011.99663411
ATAGGGG245850.011.80941138
CACCCGG133550.011.7748474
GACACGG123000.011.72397615
CGGACAC122150.011.71347113
TGGCATA246650.011.68898334
CCGGACA125400.011.55341312
ATAGCTC121750.011.51669339
GAGCTTA248650.011.43617144
CGGACAG124950.011.43298119
Produced by FastQC (version 0.11.5)