FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51477934
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT867630.1685440600627057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT185850.022.94411845
TTCGACG20000.021.5977556
AGACCGC216050.019.8476644
CAGACCG224050.019.2599813
AGAACGA240850.017.81726344
GACCGCG258300.016.6708725
TACGGAG257500.016.41093634
ATACGGA259200.016.3553933
AAGAACG266000.016.12420343
AATACGG264200.016.03494832
CGGTATA34950.016.0291420
GCGTTCT272350.015.8268699
CCCGGAT162500.015.6535291
CTCAGAC310850.015.5550211
CAATACG275700.015.55357731
ACCGCGT284400.015.2515816
CCGTACG44350.015.1676524
CGGATAC102250.015.0722963
CCGCGTT289250.014.9880717
CGTTACG23450.014.87127421
Produced by FastQC (version 0.11.5)