FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51123076
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1938370.37915754521500233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT370400.024.31642545
AGACCGC393550.023.8465184
GACCGCG399150.023.5570535
CAGACCG399950.023.5038223
ACCGCGT400650.023.4688556
CCGCGTT419250.022.4383957
CGCGTTC419800.022.398288
TTCGACG32750.022.3970586
TCAGACC446150.021.266622
AGAACGA447000.020.25109144
GCGTTCT469050.020.0970559
CTCAGAC496350.020.022781
AATACGG485000.018.76372732
CCCAATA492400.018.56999829
ATACGGA491450.018.54493933
TACGGAG491700.018.51720234
AAGAACG496850.018.34613643
CAATACG503900.018.11231431
ACTCCCC510050.018.11091225
TCGACGA40250.017.944197
Produced by FastQC (version 0.11.5)