FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52084467
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG5767161.1072706187048051No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2017980.38744372674486616No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1358480.2608224828335096No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT1350040.2592020381047578No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG1265050.24288431328288335No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC1145140.21986209439370857No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG1134260.21777317986185785No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA670870.12880423639546892No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCC641120.12309236072243956No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT605070.11617091137747458No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA597010.11462342506068075No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTT563560.1082011648501654No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGT536950.10309215605489444No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCT530840.10191906158893782No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG530840.10191906158893782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT1239500.028.930158
TTCACGC1261600.028.4394177
CACGCTC1309900.027.4062239
ACGCTCA1330200.027.1892210
GCTTCAC1384550.026.3231985
AACGCTA1400950.025.34240223
ACGCTAC1405450.025.25485624
GCCAGCT1154100.025.1946011
CGCTCAG1448250.025.00558511
AAAACGC1417600.025.00349221
CGCTACC1423050.024.99625625
GAAAACG1422100.024.87850420
AAACGCT1431400.024.82529422
GTTTTCG1451700.023.994345
CGTGGTT1512050.023.3341441
GCTCAGG1539450.023.29333312
CTACCTC1546250.023.12975127
AGAAAAC1551200.022.97473119
TCGTGGT1536800.022.9309440
TACCTCT1564000.022.8530428
Produced by FastQC (version 0.11.5)