FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52084467
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1485650.28523859138272456No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1374690.2639347350909821No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT1182490.22703313830589839No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1119490.2149374015865421No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG878770.16872016756934463No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG746090.14324616204673843No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC663720.12743146627573246No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA661760.12705515446668583No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC651630.1251102368005417No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT629310.12082489007711261No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA628810.12072889216664154No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGC612750.1176454392823104No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA544370.10451676504628529No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA543080.10426909043726991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT430500.016.6566563
GGAGGTC455150.015.5839844
GGTCACT463850.015.041177
TCAGACG232200.014.8936292
GTCACTC474400.014.692828
AGGGATC384900.014.55232052
TGGGAGG400300.014.1385222
ACTCTCC500000.014.05074211
GAGGTCA520900.013.8714235
CAGGGAT422450.013.8443151
CACTCTC514600.013.62900610
CTGGGAG448050.013.5902861
TCACTCT524550.013.566539
AGGTCAC535350.013.38501556
AGCTTAG429100.012.65198245
GTTGTCG25900.012.5156326
TGCCCGT328500.012.44594244
TATCCTA132800.012.1550265
GGCATAG445400.012.11337835
ATCCTAG143600.011.9704146
Produced by FastQC (version 0.11.5)