FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57340632
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1295170.22587299002912978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT285050.024.6159061
CCGGCTT335100.022.6589032
TCACGCT334850.022.246998
TTCACGC343850.021.7762937
ACGCTAC332800.021.0527424
ACGCTCA360600.021.0263710
AACGCTA336250.020.82319323
AGACCGC169500.020.618064
CGCTACC341400.020.42494625
GACCGCG171600.020.2738845
CACGCTC376400.020.0182679
CGGCTTC394500.019.8864333
AAAACGC355000.019.8089621
GAAAACG360950.019.46993620
CAGACCG181850.019.3787963
ACCGCGT180200.019.3554236
AAACGCT367400.019.15563422
CCGCGTT194750.017.9247657
CGTTACG24750.017.9114621
CGCGTTC201750.017.3805628
Produced by FastQC (version 0.11.5)