FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45711497
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT818640.1790884249535735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT170150.023.2324345
AGACCGC191950.020.5805954
CAGACCG199150.019.9946843
TTCGACG19550.019.44746
GACCGCG210550.018.7197675
ACCGCGT216700.018.3234546
CCGCGTT217550.018.314457
CGCGTTC219000.018.1623928
AGAACGA218900.018.0790444
ATACGGA234000.016.99045433
AATACGG235300.016.85955232
TCAGACC242800.016.6502592
TACGGAG238650.016.63115334
ACGTTCG15150.016.1858466
AAGAACG247650.015.99870743
GCGTTCT250200.015.9964469
CAATACG249750.015.9943131
CTCAGAC282400.015.6369191
ACGGAGA259350.015.32981735
CGTTACG13250.015.28443621
Produced by FastQC (version 0.11.5)