FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45711497
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG114450.014.25365345
GGCATAG126200.012.71189835
ATAGGGG131050.012.61923238
TAGCGTA4450.011.62917837
GTTGTCG17950.011.40661226
CATAGGG147050.011.10843237
TGGCATA147350.010.99417534
GAGCTTA148950.010.89177644
ACCCGGC69850.010.8231725
ATAGCTC63450.010.74468339
GGGAGGT230700.010.5916573
GGTCACT219250.010.4264227
GCATAGG156400.010.40118236
ACTCTCC231950.010.01074811
GGAGGTC247800.09.8516764
GTCACTC243850.09.6329428
TAGCTCT71600.09.45884240
TCAGACG70450.09.4534632
CACTCTC256050.09.07731110
TCACTCT267500.09.0167959
Produced by FastQC (version 0.11.5)