FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45742787
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG17650.010.19824626
ATAGGGG74550.08.69220238
AGCTTAG78150.08.2341245
CGTAACG9900.08.18177442
GGCATAG78050.07.95648335
ACCCGGC53150.07.9162325
GGGAGGT146950.07.7168693
TCACCCG62800.07.7029853
GTAACGA30600.07.499960418
CATAGGG87350.07.31543937
GCATAGG88150.07.274573336
CGTAGGG40250.07.26712237
GCGTAGG40000.07.25629136
TAATCGG34450.07.1842874
GCGTTCG11050.07.12665915
GGCGTAG40500.07.11115135
CGGACAC83700.06.935446713
CTAGAAT68800.06.86769631
CGGCCCG71750.06.8675598
ATATAGC40800.06.83819966
Produced by FastQC (version 0.11.5)