FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58577571
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG168600.014.89574345
ATAGGGG179850.013.83680138
GGCATAG182450.013.55099135
GGGAGGT306050.012.3558913
GTTGTCG28250.012.262753526
GGTCACT292200.012.1288177
GAGCTTA207000.012.04550444
GTCACTC299750.011.9435328
CATAGGG215550.011.72187737
TGGCATA210700.011.71268234
ACTCTCC310400.011.52630711
GGAGGTC337700.011.1378344
GCATAGG225900.011.12427236
GAGGTCA347350.011.0550985
TCACTCT350350.010.4048239
CACTCTC353400.010.14307310
TAGGGGA372250.09.59320439
CGTAGGG112750.09.49851837
GGCGTAG111950.09.46485735
GCGTAGG113150.09.40461936
Produced by FastQC (version 0.11.5)