FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49750725
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1339890.26932069834158195No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT750700.15089227342918118No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG676290.13593570746958963No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT554380.1114315419524037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT314850.021.3035241
AGACCGC187350.020.9447844
TTCGACG20600.020.424136
CAGACCG195050.020.2679063
CCGGCTT374000.020.2142982
GACCGCG199450.019.6064455
ACCGCGT210950.018.600996
TCACGCT394300.018.579198
TTCACGC399800.018.3686187
CGGCTTC444750.017.7369563
CCGCGTT222500.017.6455257
CGCGTTC227600.017.2402428
ACGCTCA444000.016.62617910
TCAGACC245300.016.5474072
CACGCTC462450.015.9044739
TCGACGA26300.015.5698637
GCGTTCT254250.015.4685519
CTCAGAC291600.015.4556251
GTCCGTT37500.015.4203791
GAACGAT255200.015.28753945
Produced by FastQC (version 0.11.5)