FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49750725
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG676400.13595781770014406No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA672070.13508747862468337No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG600090.12061934775824876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT278750.014.6233953
ATAGGGG235000.013.27068238
GGAGGTC307050.013.2536574
AGCTTAG236850.013.22640845
GGCATAG237900.013.09658335
GTTGTCG22250.012.83917426
GGTCACT321100.012.1731467
GAGCTTA261350.012.08128244
TGGCATA259800.011.97531834
AGGGATC205300.011.9286812
GTCACTC334650.011.7884138
TCAGACG100150.011.3499232
TGGGAGG292100.011.3044922
ACTCTCC352650.011.2245911
CAGGGAT248450.010.9174141
CATAGGG292800.010.60456537
CGTGGCG155400.010.4582241
TCACTCT389750.010.3643639
CACTCTC386450.010.2315810
GCATAGG307250.010.1267536
Produced by FastQC (version 0.11.5)