FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42082859
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1074720.25538188838358156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT194350.025.90951745
AGACCGC225050.023.244464
CAGACCG231550.022.7182163
GACCGCG238650.021.9858445
ACCGCGT242300.021.6917936
AGAACGA234300.021.54925344
TTCGGCG65300.021.29398744
CGCGTTC247200.021.2891248
CCGCGTT249700.021.0669657
GCGTTCT273150.019.348979
AAGAACG263000.019.24032243
AATACGG271050.019.21648832
TCAGACC278900.019.1354452
ATACGGA272350.019.13302433
TACGGAG273650.018.98457534
TCGGCGC75550.018.55399345
CAATACG281350.018.50499231
CCGTACG74900.018.5043754
CGTACGC74950.018.4620325
CTCAGAC319800.017.8225211
Produced by FastQC (version 0.11.5)