FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50324274
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT614320.12207230252343035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC147250.020.7824
GACCGCG154050.019.8938665
CAGACCG156600.019.6705043
ACCGCGT157850.019.4092986
CGCGTTC174300.018.8164398
AACGCTA164450.018.43890423
CCGCGTT166350.018.3904957
ACGCTAC153900.017.466624
GAAAACG169350.017.16154520
AAAACGC181450.016.86013221
CGCTACC159700.016.76175125
TCACGCT195400.016.5773568
ACGCTCA206950.016.39129410
GATCGCG13600.016.0485785
TTCACGC205900.015.7756837
CGTTACG20300.015.73516721
TTCGGCG86950.015.54827444
CCGTACG89450.015.3445194
AAACGCT200400.015.29949822
TCAGACC205750.015.1819222
Produced by FastQC (version 0.11.5)