FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53460407
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG657560.12299943769601306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG20550.020.5839546
AGACCGC93450.017.9614724
TCGACGA27000.016.8333597
GCCGGCT253450.016.7714581
ACGAACG69500.016.70481131
CCGGCTT289050.016.63482
GACCGCG101100.016.5356045
CAGACCG104100.016.4049573
CGTTACG23650.016.3633721
CGAACGA71900.016.02203832
TTCACGC288850.015.7737837
GTTCGAC29400.015.4591785
ACCGCGT111500.015.1547176
CCCGGAT111500.015.0151331
TCACGCT304150.014.8918178
CTCCGAT47800.014.7349121
CCCGTAT35950.014.646981
ACGCTCA314800.014.58842910
GTCCGTT39450.014.5453531
AACGAAC85050.013.94161230
Produced by FastQC (version 0.11.5)