FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53460407
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG35400.013.79260726
ATAGGGG136150.011.80244838
GGCATAG141350.011.51034935
GGGAGGT199850.010.749253
AGCTTAG151500.010.6508445
ATATAGC57450.010.1412996
GGAGGTC214250.09.6594154
GAGCTTA166000.09.59898344
GCATAGG169350.09.59390136
TGGCATA171800.09.49635234
CATAGGG169900.09.47047337
GACGCAA58450.09.39518644
GTCGTTC50750.09.35553129
TGTCGTT50700.09.32057628
TTGTCGT55200.09.29463727
AGGGATC170300.09.272592
TCGTTCC54450.09.05090230
ATAATCG57950.09.0444353
GTAACGA49100.09.02656818
GCTAAGT53800.08.94865114
Produced by FastQC (version 0.11.5)