FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51260672
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2922790.5701817564935551No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG689970.13460026431179054No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA683990.13343367796660957No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG563400.10990882054765104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT479800.029.62875445
AGAACGA570850.024.93053844
GACCGCG581950.024.552265
ACCGCGT583000.024.496186
AGACCGC587700.024.358244
CCGCGTT596900.023.9445827
CAGACCG600100.023.94063
CGCGTTC598200.023.8925468
TTCGACG24450.023.3714056
TCAGACC637300.022.747492
GCGTTCT643900.022.2675259
AAGAACG649250.021.9515243
CTCAGAC696700.021.7175851
AATACGG675600.021.04875232
ATACGGA683000.020.83625833
TACGGAG683350.020.79253434
CAATACG697250.020.4571831
CGTTCTC722800.020.10410510
CGGAGAG708850.019.9775536
ACGGAGA714400.019.92330235
Produced by FastQC (version 0.11.5)