FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51260672
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG789400.1539972008170318No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA788330.15378846379540245No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG528540.10310828543176336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG174550.014.57823645
ATAGGGG179200.014.51326338
GGCATAG177100.014.49447635
GTTGTCG24350.013.305799526
GAGCTTA196900.012.93473144
TGGCATA200150.012.8364734
CATAGGG200650.012.79359337
GGGAGGT266350.012.6935353
AGGGATC191600.012.5988492
GCGTTCG27550.012.4958315
CGCGTTC27850.012.11760514
GCATAGG214300.012.09367236
GGAGGTC280550.012.054264
CGTTCGC28450.011.78424616
CCGCGTT29900.011.66304913
GTCACTC294550.011.4106758
GGTCACT296500.011.176127
ACTCTCC316200.010.5580211
CAGGGAT254550.010.5573971
CCTAGGC98500.010.0036328
Produced by FastQC (version 0.11.5)