FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47275779
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1334170.2822100509438459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT265950.023.85155545
GACCGCG299250.021.237545
ACCGCGT300050.021.1943176
AGACCGC301700.021.1321854
CAGACCG309850.020.6706663
CCGCGTT311350.020.42517
CGCGTTC313550.020.2674648
AGAACGA319300.019.9086544
TCAGACC353800.018.433782
GCGTTCT354600.018.029069
TTCGGCG47350.017.83368544
AAGAACG357400.017.73291643
CTCAGAC394900.017.4877681
TACGGAG366350.017.21105834
ATACGGA369250.017.1668133
AATACGG370350.017.1153532
CCGTACG52750.016.3341374
CGTACGC53550.016.258165
CAATACG392800.016.25092331
ACGGAGA393800.016.04033335
Produced by FastQC (version 0.11.5)