FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49424054
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2451430.49599937714538755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT418750.027.9436145
AGACCGC464200.027.32244
CAGACCG470950.027.0167923
GACCGCG485500.026.0402855
ACCGCGT490850.025.7862536
CCGCGTT500000.025.29687
CGCGTTC503700.025.0928848
AGAACGA473750.024.77073144
GCGTTCT519550.024.3403639
TCAGACC532650.024.2266252
CTCAGAC561350.023.791981
TACGGAG525150.022.32399634
AATACGG527950.022.26561732
ATACGGA528250.022.24005933
AAGAACG538500.021.88417843
CGTTCTC596800.021.51770810
CAATACG554050.021.30613531
ACGGAGA554850.021.16010935
CCCAATA568550.020.83002529
CGGAGAG563300.020.75479336
Produced by FastQC (version 0.11.5)