FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49424054
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG131450.013.44816545
ACCCGGC81800.013.2025725
GGCATAG135250.012.87062135
ATAGGGG140250.012.50826838
GTTGTCG21550.012.00311526
ATAGCTC84850.011.90121339
GAGCTTA152550.011.78027644
GGGAGGT273200.011.4399333
TCACCCG103450.011.3538173
TAGCTCT92700.011.2086840
TGGCATA159200.011.07563934
CATAGGG162450.010.93763437
AGGGATC157000.010.7632292
GGTCACT260950.010.5624747
TAGCGTA7250.010.5476837
ACTCTCC264450.010.52560311
GTCACTC269350.010.4005538
GCATAGG177600.010.02993936
GGAGGTC286550.010.027264
CCGGACA123600.09.98699312
Produced by FastQC (version 0.11.5)